Sample: MMETSP1421


Sample Name MMETSP1421 
Sample Type eukaryotes
Project Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP)
Investigators (1)
URL N/A
Description

Thalassiosira weissflogii CCMP1010

Sample Accession CAM_SMPL_003189
Assembly  
Combined Assembly Thalassiosira-weissflogii-CCMP1010
Taxon ID 67004

Ph 8.2  
Phosphate 36.2  
Nitrate 882  
Silicate 10.6  
Axenic 1  
Trace elements 1093.8  
Experimental salinity 35  

Longhurst province NASW  

Growth medium f/2 amended seawater  
Modifications to growth medium f/2 for all nutrients except Fe added at 4nM; Cultures preacclimated; After onset of FeLim, 10nM Fe Added  

NCBI SRA SRS616890  

Habitat name marine habitat  
Habitat marine habitat  

Latitude 37  
Longitude -65  

Project id 104  
Collection start time 1969-12-01 00:00:00.0  
Collection stop time 1969-12-01 00:00:00.0  
Clonal 1  
Collection date 01-DEC-69  
Date of experiment 15-MAR-13  
External sample id CCMP1010_+24HrFeAddB  
Investigation type EU  
Principle investigator Bethany Jenkins  
Additional citations Mock, T. et al. Whole-genome expression profiling of the marine diatom Thalassiosira pseudonana identifies genes involved in silicon bioprocesses. P Natl Acad Sci Usa 105, 1579-1584 (2008).  
Primary citation von Dassow, P., Petersen, T. W., Chepurnov, V. A. & Armbrust, E. V. Inter- and intraspecific relationships between nuclear DNA content and cell size in selected members of the centric diatom genus Thalassiosira (Bacillariophyceae). J Phycol 44, 335-349 (  
Primary citation von Dassow, P., Petersen, T. W., Chepurnov, V. A. & Armbrust, E. V. Inter- and intraspecific relationships between nuclear DNA content and cell size in selected members of the centric diatom genus Thalassiosira (Bacillariophyceae). J Phycol 44, 335-349 (2  
Other experimental metadata available Sampled 24 hrs after 10nM Fe addition to Fe Lim (4nM Fe) culture. Trace elements: 39.3 nM Cu; 26 nM Mo; 76.5 nM Zn; 42 nM Co; 910 nM Mn; 10-14 nM Fe; EDTA = 11.7 uM  

GenBank BioSample 2740018  
Fastq file /iplant/home/shared/imicrobe/projects/104/transcriptomes/MMETSP142/MMETSP1421.fastq.tar  

Day portion of day night cycle in hours 14  
Light 120  
Experimental temperature 19  
Night portion of day night cycle in hours 10  

Library acc CAM_LIB_001935  
Sequencing method Illumina  
Dna type cDNA  
Assembly accession number CAM_ASM_000907  
Combined assembly name Thalassiosira-weissflogii-CCMP1010  
Pcr amp No  

Source mat id (MMETSP1421) Thalassiosira weissflogii - seawater  
Source mat id CAM_BM_003209  
Source mat id CAM_SMPL_003189  
Sample type eukaryotes  
Sample description Thalassiosira weissflogii CCMP1010  
Sample name MMETSP1421  
Taxon id 67004  
Sample material seawater  

Strain CCMP1010  
Genus Thalassiosira  
Species weissflogii  
Family Skeletonemaceae  
Superkingdom Eukaryota  
Class Coscinodiscophyceae  
Phylum Bacillariophyta  

N/A