Sample: MMETSP0922


Sample Name MMETSP0922 
Sample Type eukaryotes
Project Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP)
Investigators (1)
URL N/A
Description

Perkinsus marinus ATCC 50439

Sample Accession CAM_SMPL_002782
Assembly  
Combined Assembly Perkinsus-marinus-ATCC50439
Taxon ID 1032511

Ph 7  
Axenic No  
Experimental salinity 29  
Environmental salinity 12  

Growth medium 850 mOsm/kg DME:Ham's F-12 (Burreson et al. 2005, J. Eukaryot. Microbiol. 52:258-270)  
Modifications to growth medium 3% (v/v) FBS, 100 µg/ml streptomycin, 100 U/ml penicillin  

NCBI SRA SRS616850  

Habitat name organism-associated habitat  
Habitat organism-associated habitat  
Habitat description Benthic estuarine oyster reef  

Site name Chesapeake Bay  
Latitude 38.71306  
Longitude -76.32083  
Longitude 76.32083  
Site description Choptank River, Tilghman Wharf, Maryland  
Country UNITED STATES  
Country US  
Depth 4  
Sample collection site Chesapeake Bay  
Other collection site info Choptank River, Tilghman Wharf, Maryland  

Project id 104  
Collection start time 1992-10-20 00:00:00.0  
Collection stop time 1992-10-20 00:00:00.0  
Clonal 1  
Collection date 20-OCT-92  
Date of experiment 13-MAY-11  
External sample id Permaexp  
Investigation type EU  
Principle investigator Senjie Lin  
Additional citations Dungan and Hamilton 1995, J. Eukaryot. Microbiol. 42:379-388  
Primary citation Zhang, H., Campbell, D. A, Strum, N. R., Dungan, C. F. and Lin, S. 2011. Spliced leader RNA, mitochondrial gene frameshifts, and multi-protein phylogeny expand support for Perkinsus as a unique alveolate. PLoS ONE 6(5): e19933. Doi:10.1371/journal.pone.0  
Primary citation Zhang, H., Campbell, D. A, Strum, N. R., Dungan, C. F. and Lin, S. 2011. Spliced leader RNA, mitochondrial gene frameshifts, and multi-protein phylogeny expand support for Perkinsus as a unique alveolate. PLoS ONE 6(5): e19933. Doi:10.1371/journal.pone.00  
Other experimental metadata available submitted is Perkinsus spliced leader based cDNA prepared when culture was in exponential phase.  

GenBank BioSample 2740110  
Fastq file /iplant/home/shared/imicrobe/projects/104/transcriptomes/MMETSP092/MMETSP0922.fastq.tar  

Pressure 1  
Experimental temperature 27  
Environmental temperature 13  

Library acc CAM_LIB_001548  
Sequencing method Illumina  
Dna type cDNA  
Assembly accession number CAM_ASM_000600  
Combined assembly name Perkinsus-marinus-ATCC50439  
Pcr amp 1  

Source mat id (MMETSP0922) Perkinsus marinus - Crassostrea virginica host-oyster  
Source mat id 3258  
Source mat id CAM_BM_002803  
Source mat id CAM_SMPL_002782  
Sample type eukaryotes  
Sample description Perkinsus marinus ATCC 50439  
Sample name MMETSP0922  
Sample name MMETSP0922C  
Taxon id 1032511  
Sample material Crassostrea virginica host-oyster  

Strain ATCC 50439  
Genus Perkinsus  
Species marinus  
Family Perkinsidae  
Order Perkinsida  
Superkingdom Eukaryota  
Class Perkinsea  
Phylum Perkinsozoa  

N/A