Sample: MMETSP0924C


Sample Name MMETSP0924C 
Sample Type eukaryotes
Project Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP)
Investigators (1)
URL N/A
Description

Perkinsus chesapeaki ATCC PRA-65 (combined sample which includes MMETSP0924 and MMETSP0925)

Sample Accession CAM_SMPL_002745
Assembly  
Combined Assembly Perkinsus-chesapeaki-ATCC_PRA_65
Taxon ID 321610

Ph 7  
Axenic No  
Experimental salinity 29  
Environmental salinity 13.5  

Growth medium 850 mOsm/kg DME:Ham's F-12 (Burreson et al. 2005, J. Eukaryot. Microbiol. 52:258-270)  
Modifications to growth medium 3% (v/v) FBS, 100 µg/ml streptomycin, 100 U/ml penicillin  

NCBI SRA SRS621313  

Habitat name organism-associated habitat  
Habitat organism-associated habitat  
Habitat description The host clam was infaunal within a benthic sand and mud substrate from which it was excavated.  

Site name Chesapeake Bay  
Latitude 38.9944  
Longitude -76.2141  
Longitude 76.2141  
Site description Chester River, Buoy Rock, Maryland  
Country UNITED STATES  
Country US  
Depth 4  
Sample collection site Chesapeake Bay  
Other collection site info Chester River, Buoy Rock, Maryland  

Project id 104  
Collection start time 2002-08-27 00:00:00.0  
Collection stop time 2002-08-27 00:00:00.0  
Clonal 1  
Collection date 27-AUG-02  
Date of experiment 13-MAY-11  
External sample id Perchexp  
Investigation type EU  
Principle investigator Senjie Lin  
Additional citations Burreson et al. 2005, J. Eukaryot. Microbiol. 52:258-270.  
Primary citation Zhang, H., Campbell, D. A, Strum, N. R., Dungan, C. F. and Lin, S. 2011. Spliced leader RNA, mitochondrial gene frameshifts, and multi-protein phylogeny expand support for Perkinsus as a unique alveolate. PLoS ONE 6(5): e19933. Doi:10.1371/journal.pone.0  
Primary citation Zhang, H., Campbell, D. A, Strum, N. R., Dungan, C. F. and Lin, S. 2011. Spliced leader RNA, mitochondrial gene frameshifts, and multi-protein phylogeny expand support for Perkinsus as a unique alveolate. PLoS ONE 6(5): e19933. Doi:10.1371/journal.pone.00  
Other experimental metadata available submitted is Perkinsus spliced leader based cDNA prepared when culture was in exponential phase.  

GenBank BioSample 2740203  
Fastq file /iplant/home/shared/imicrobe/projects/104/transcriptomes/MMETSP092/MMETSP0924.fastq.tar  

Pressure 1  
Experimental temperature 27  
Environmental temperature 27.8  

Library acc CAM_LIB_001511  
Sequencing method Illumina  
Dna type cDNA  
Assembly accession number CAM_ASM_000563  
Combined assembly name Perkinsus-chesapeaki-ATCC_PRA_65  
Pcr amp 1  

Source mat id (MMETSP0924) Perkinsus chesapeaki - Mya arenaria host clam  
Source mat id CAM_BM_002765  
Source mat id CAM_SMPL_002745  
Sample type eukaryotes  
Sample description Perkinsus chesapeaki ATCC PRA-65 (combined sample which includes MMETSP0924 and MMETSP0925)  
Sample name MMETSP0924C  
Taxon id 321610  
Sample material Mya arenaria host clam  

Strain ATCC PRA-65  
Genus Perkinsus  
Species chesapeaki  
Family Perkinsidae  
Order Perkinsida  
Superkingdom Eukaryota  
Class Perkinsea  
Phylum Perkinsozoa  

N/A