Sample: MMETSP0955


Sample Name MMETSP0955 
Sample Type eukaryotes
Project Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP)
Investigators (1)
URL N/A
Description

Aplanochytrium sp PBS07

Sample Accession CAM_SMPL_002681
Assembly  
Combined Assembly Aplanochytrium-sp-PBS07
Taxon ID 627963

Ph 8  
Axenic 1  
Experimental salinity 25  

Longhurst province NWCS  

Growth medium artificial seawater with 1% horse serum (0.8% agar plates)  
Modifications to growth medium artificial seawater (25 grams per liter Instant Ocean) plus 0.8% agar autoclaved, then filter sterilized horse serum (1% vol:vol) added just before pouring plates  
Other environmental metadata available none available  

NCBI SRA SRS616872  

Habitat name marine habitat  
Envo term for habitat primary term Acquatic: marine  
Envo term for habitat secondary term Other  
Habitat marine habitat  
Habitat description surface sediment in Peconic Bay, Long Island, New York, USA  

Site name OTHER  
Latitude 40.93  
Longitude -72.5  
Country UNITED STATES  
Country US  
Depth 7  
Sample collection site OTHER  

Project id 104  
Collection start time 2007-10-29 00:00:00.0  
Collection stop time 2007-10-29 00:00:00.0  
Clonal 1  
Collection date 29-OCT-07  
Date of experiment 26-MAR-12  
External sample id Aplanochytrium PBS07 horse serum  
Investigation type EU  
Principle investigator Jackie Collier  
Additional citations Collado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Damare, V. & Raghukumar, S. 2010. Association of the  
Additional citations Collado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Damare, V. & Raghukumar, S. 2010. Association of the s  
Primary citation Leander CA, Porter D, Leander BS (2004) Comparative morphology and molecular phylogeny of aplanochytrids (Labyrinthulomycota). Europ. J. Protistol. 40:317-328.  
Other experimental metadata available Harvested 275 mg (wet weight) biomass from 40 plates after 19 to 20 days of growth  

GenBank BioSample 2740265  
Fastq file /iplant/home/shared/imicrobe/projects/104/transcriptomes/MMETSP095/MMETSP0955.fastq.tar  

Pressure 1  
Experimental temperature 23  
Environmental temperature 13  

Library acc CAM_LIB_001447  
Sequencing method Illumina  
Dna type cDNA  
Assembly accession number CAM_ASM_000499  
Combined assembly name Aplanochytrium-sp-PBS07  
Pcr amp No  

Source mat id (MMETSP0955) Aplanochytrium sp - sediment (surface 0 to 2 cm)  
Source mat id 3156  
Source mat id CAM_BM_002701  
Source mat id CAM_SMPL_002681  
Sample type eukaryotes  
Sample description Aplanochytrium sp PBS07  
Sample name MMETSP0955  
Taxon id 627963  
Sample material sediment (surface 0 to 2 cm)  

Strain PBS07  
Genus Aplanochytrium  
Species sp  
Family Thraustochytriaceae  
Order Labyrinthulomycetes  
Superkingdom Eukaryota  
Class Labyrinthulea  
Phylum Labyrinthista  

N/A