Project Name A gnotobiotic mouse model of phage-bacterial host dynamics in the human gut 
Domain (1) viruses
Investigators (0) N/A
Institution WUSL
Project Code PRJEB4370
Project Type metagenome


The microbial diversity, interpersonal variation and dynamism of the human gut microbiota make the task of identifying the factors that define community configurations, both structural and functional, extremely challenging. Bacterial viruses (phage) are the most abundant biological group on Earth and are more genetically diverse than their bacterial prey/hosts. To characterize their role as agents shaping gut community structure, adult germ-free mice were colonized with a consortium of 15 sequenced human bacterial symbionts, 13 of which harbored one or more predicted prophage in their genomes. One member, Bacteroides cellulosilyticus WH2, was represented by a library of isogenic transposon mutants covering 90% of its genes. Once assembled, the community was subjected to a staged phage attack with a pool of live or heat-killed virus-like particles (VLPs) purified from the fecal microbiota of five healthy humans. Shotgun sequencing of DNA from the input pooled human VLP preparation plus shotgun sequencing of gut microbiota samples, and purified fecal VLPs, from the gnotobiotic mice revealed a reproducible non-simultaneous pattern of attack extending over a 25 day period and involving five phage, none described previously. This system allowed us to (i) correlate increases in abundances of specific phage present in the input VLP preparation with reductions in the representation of particular bacterial hosts, (ii) provide evidence that phage resistance occurred because of ecological or epigenetic factors, (ii) track the origin of each of the five phages among the five human donors plus the extent of their genome variation between and within recipient mice, and (iv) establish the dramatic in vivo fitness advantage that a locus within a B. cellulosilyticus prophage confers upon its host bacterium. Together, these results provide a defined community-wide view of phage-bacterial host dynamics in the gut ecosystem.


No assemblies.


Type Location
Meta  /iplant/home/shared/imicrobe/projects/132/sample-attr.tab
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2936/ERR346639.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2936/ERR346651.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2920/ERR346640.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2938/ERR346641.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2932/ERR346642.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2933/ERR346643.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2914/ERR346626.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2921/ERR346644.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2921/ERR346652.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2922/ERR346645.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2909/ERR346608.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2909/ERR346611.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2906/ERR346609.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2906/ERR346612.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2923/ERR346646.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2923/ERR346653.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2908/ERR346614.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2911/ERR346615.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2929/ERR346616.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2903/ERR346617.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2937/ERR346647.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2937/ERR346654.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2927/ERR346618.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2912/ERR346619.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2928/ERR346620.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2924/ERR346648.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2924/ERR346657.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2905/ERR346621.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2931/ERR346622.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2934/ERR346627.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2930/ERR346623.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2913/ERR346624.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2907/ERR346625.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2907/ERR346649.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2915/ERR346628.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2939/ERR346629.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2917/ERR346631.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2916/ERR346630.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2926/ERR346632.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2926/ERR346655.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2918/ERR346633.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2925/ERR346634.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2925/ERR346650.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2925/ERR346656.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2935/ERR346635.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2904/ERR346636.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2902/ERR346637.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2919/ERR346638.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2910/ERR346610.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2910/ERR346613.fasta
Reads  /iplant/home/shared/imicrobe/projects/132/samples/2901/ERR346555.fasta